Sortilin, R&D Systems, AF3154

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Hu et al. Frontiers in Neuroanatomy, 2017.
Hu et al. validate this antibody for detection of mice sortilin by Western blotting and immunolabeling of mouse brain lysate and tissue, respectively. This antibody targets the extracellular domain in sortilin from +/+ and -/- brains. No band was observed in the -/- mouse brain WB, and no labeling was seen in the -/- (KO mice) immunolabeling (figure 1B-C).
Based on the available data, we have assigned a five-star rating in both the Western blot and the immunolabeling, but we highly encourage other scientists to share their observations.

The antibody detects both human and murine sortilin in IHC.
Dilution 1:100

This antibody work great with immunostaning of human brain tissue (Hippocampus region).

Dilution 1:250

This antibody cannot be used for IP of sortilin from HEK293 cells after treatment with crosslinking reagent DSP