Transferrin receptor, Abcam, ab84036
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User Reviews (3)
Review of: Transferrin receptor, Abcam, ab84036 by Andrea E. Toth, Aarhus University
This antibody works well for immunofluorescence staining in mouse (b.End3), human (hCMEC/D3) brain endothelial cell line and in primary porcine brain endothelial cells. Dilution 1:200. Fixation was done with 4% paraformaldehyde or methanol cells were permeabilized with 0.25% Triton X-100.
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0 not usefulReview of: Transferrin receptor, Abcam, ab84036 by Niels M Kristiansen
Producer: Abcam
Catalog No: ab84036
Used dilution: 1:1000 (WB)
Secondary Ab: Anti-Rabbit HRP, Cell signaling 7074 (1:2000)
Cell line: primary porcine brain endothelial cells, HEK 293
Lysate, Protein Concentration: 5 ug
Host organism: Rabbit
Antibody tested with primary porcine brain endothelial and HEK 293 cell lysis.
Both pBEC and HEK 293 produced clear nice bands at the appropriate size.
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0 useful 0 not usefulReview of: Transferrin receptor, Abcam, ab84036 by Simon Glerup, Aarhus
I have tested it as marker of endocytic compartments in HEK293 cells.
Nice vesicular-like staining in immunofluorescense. Seems very specific.
Dilution 1:200.
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