Tyrosine hydroxylase, BD Bioscience, 612300
What do reviewers generally think about 612300 from BD Bioscience?
Researchers report the antibody performs well for immunofluorescence of mouse dopaminergic neurons, including ES-cell-derived neurons and primary neurons, with clear cytoplasmic and neurite staining, and for Western blotting on mouse tissues. It also works in immunohistochemistry on mouse brain after anti-mouse Fab blocking, though one reviewer points out that some background staining remains despite blocking. Recommended dilutions are 1:500 for one IF protocol, 1:1000 for IF/IHC, and 1:2000 for Western blotting; it also supported double staining with rabbit anti-GABA.
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Review of: Tyrosine hydroxylase, BD Bioscience, 612300 by Urs Kindler
The antibody was used for Immunofluorescence staining of neurons derived from murine ES cells differentiated in culture to investigate the presents of dopaminergic neurons.
The cells were grown on coated cover slips, fixed for 30min in 4% PFA and treated for 5 min with 0,005% Triton-X-100. To reduce unspecific binding cells are blocked/ quenched with a 1% BSA in PBS solution.
The Antibody was diluted 1:500. The secondary antibody was a goat anti mouse 488 diluted 1:1000.
A clear and strong staining of the cytoplasm into the neurites was observed. The antibody worked well in double staining experiments with Sigma # A2052 Rabbit-anti-GABA (1:250).
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0 not usefulReview of: Tyrosine hydroxylase, BD Bioscience, 612300 by Simon Glerup, Aarhus
Excellent antibody for Western blotting.
We have used it on a number of mouse tissues.
It also works for IF staining of primary mouse dopaminergic neurons.
Also works for immunohistochemistry on mouse brain tissues following blocking with anti-mouse Fab fragments. Some background staining still remains following blocking.
IF and IHC 1:1000
WB 1:2000
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