Alpha-synuclein, Academia, Academia-0010
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Researchers report that Alpha-synuclein, Academia, Academia-0010 works very well for western blotting, immunohistochemistry, immunoprecipitation, ELISA capture, and reprobing old membranes, using dilutions of 1:500 to 1:1000 and 5 ug per cell lysate for IP. One reviewer points out that for western blotting, fixing the membrane in 4% PFA for 30 min and boiling in PBS for 5 min before milk blocking optimizes detection. No specific complaints are noted.
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Review of: Alpha-synuclein, Academia, Academia-0010 by Rikke H Kofoed, Aarhus University
I routinely use this antibody for western blotting (1:500), immunohistochemistry (1:500), immunoprecipitation (5 ug pr. cell lysate from a densely seeded well of a 24 wells plate) and ELISA (as capture antibody – used 1 ug/uL for coating of a maxisorp plate). It works very well for all four purposes.
NB: When performing western blotting of alpha-synuclein I will recommend a fixation of the membrane in 4% PFA for 30 min followed by boiling in PBS for 5 min before blocking in milk to optimize detection.
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0 not usefulReview of: Alpha-synuclein, Academia, Academia-0010 by Simon Glerup, Aarhus
Excellent antibody produced in Professor Poul Henning Jensens lab, Aarhus University.
We have used it for immunohistochemistry on PFA-fixed cryosection of mouse brain. Dilution 1:1000.
And for Western blotting of mouse hippocampal homogenates. Dilution 1:1000. It also works for reprobing of old membranes.
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