Sortilin, Sigma-Aldrich, MABN1792
What do reviewers generally think about MABN1792 from Sigma-Aldrich?
Researchers report that Sortilin MABN1792 performs well for ELISA detection of soluble sortilin, including human plasma, and for immunostaining, IF, or IHC of endogenous sortilin in mouse cells and tissues, often at 1:100 or 0.05 to 1 ug/ml. It was also used after AF647 conjugation for flow cytometry on murine cells. One reviewer points out that it failed for IHC on mouse DRG when used with Fab fragments. Another notes it is not reactive against rat sortilin.
Information about this antibody
Vendor notes
User Reviews (8)
Review of: Sortilin, Sigma-Aldrich, MABN1792 by Ditte Køster
Conjugated to AF647 and then used for flow cytometry to stain murine lung single cell suspension, murine splenocytes and murine blood cells.
Stained in 100 uL, 20 min, RT, 1:100
“Reshaping the progranulin/sortilin interaction for targeted degradation of extracellular proteins”
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0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Laura Johnsen
I have used it for IHC on mouse DRG (dorsal root ganglia), at a dilution of 1:100, in combination with Fab-fragments, without success! I could not get it to work properly.
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Thaneas Prabakaran, Aarhus University
It works very well for immunostaining of PFA fixed cells at a dilution of 1:100
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Stine Klinger
Not reactive against rat sortilin
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Camilla Gustafsen
We have used this antibody as detection antibody (1 µg/ml) in a sortilin specific ELISA used for quantification of soluble sortilin in human plasma. As capture antibody we used pAb anti-sortilin/F7615 (10 µg/ml).
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Camilla Gustafsen
This is a very good antibody for immunostaining of endogenous sortilin in HepG2 cells. Gives nice staining at concentration 1 μg/ml.
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Simon Glerup, Aarhus
Great sortilin antibody for detection in ELISA.
Dilution 0.05 to 1 ug/ml
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0 useful 0 not usefulReview of: Sortilin, Sigma-Aldrich, MABN1792 by Anja A, Aarhus
This mAb works for the detection of mouse sortilin by IF in cultured cerebellar granule neurons and by IHC on brain sections.
Dilution 1:100
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