Sortilin, Academia, F7615
What do reviewers generally think about F7615 from Academia?
Researchers generally report that Sortilin, Academia, F7615 performs well for immunostaining/immunofluorescence of endogenous sortilin in HEK293, SY5Y, HepG2, and patient-derived HDFs, often at 1:200 or 1:1000 serum dilution. It is also described as useful for IP/pull-down of endogenous sortilin from HepG2 lysate, as an ELISA capture antibody for soluble human plasma sortilin, and for Western blotting of human sortilin at 1:1000. One reviewer points out that it was not specific and not useful for IHC on cryosectioned mouse dorsal root ganglia at 1:1000.
User Reviews (7)
Review of: Sortilin, Academia, F7615 by Lasse Voss
It works great for immunostaining of sortilin in both HEK293 cells and patient-derived HDFs. Dilution: 1:200.
0 useful
0 not usefulReview of: Sortilin, Academia, F7615 by Laura Johnsen
I have used this antibody for IHC on cryosectioned mouse dorsal root ganglia, on both WT and KO. It was not specific, and was not useful!
Dilution: 1:1000
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0 useful 0 not usefulReview of: Sortilin, Academia, F7615 by Camilla Gustafsen
This is a very good antibody for IP. We have used it for pull down of endogenous sortilin from HepG2 cell lysate after cross-linking of cellular proteins with DSP.
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0 useful 0 not usefulReview of: Sortilin, Academia, F7615 by Camilla Gustafsen
This antibody works very well as capture antibody (10 µg/ml) for ELISA detection of soluble sortilin in human plasma. As detection antibody we used mAb anti-sortilin/F11 (1 µg/ml)
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0 useful 0 not usefulReview of: Sortilin, Academia, F7615 by Camilla Gustafsen
We have used this antibody for IF detection of endogenous sortilin in HepG2 cells with very good results. Serum was diluted 1:1000
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0 useful 0 not usefulReview of: Sortilin, Academia, F7615 by Simon Glerup, Aarhus
This antibody is from Professor Claus Munck Petersen lab, Aarhus University.
Excellent for immunostaining of sortilin in 293 and SY5Y cells.
Dilution 1:200 of serum (5438 vial).
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0 useful 0 not usefulReview of: Sortilin, Academia, F7615 by Anja A, Aarhus
Works great for Western blotting for detection of human sortilin.
Dilution of serum 1:1000
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0 useful 0 not useful
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