SorCS2, Academia, Academia-0006
What do reviewers generally think about Academia-0006 from Academia?
Researchers generally find SorCS2, Academia, Academia-0006 useful for detecting human SorCS2, especially two-chain forms, in immunofluorescence, IP, and ELISA, with good staining in HEK293 cells and neurons. One reviewer points out that it binds two-chain SorCS2 better than one-chain and gives only okay IP performance. It is less reliable for soluble purified SorCS2 ELISA, with specific but low signal as detector and mixed, low-sensitivity capture results, and it does not substantially IP murine SorCS2.
User Reviews (7)
Review of: SorCS2, Academia, Academia-0006 by Sune Skeldal, Aarhus University
This antibody gives a specific but low signal as detector antibody in ELISA (1 µg/mL) detecting soluble purified SorCS2 in combination with rabbit anti-human SorCS2 extracellular domain (F7100) as capture antibody.
0 useful
0 not usefulReview of: SorCS2, Academia, Academia-0006 by Sune Skeldal, Aarhus University
For detecting purified soluble SorCS2 I used this antibody as capture antibody (10 µg/mL) in ELISA with mixed results. It is not super good but may be used if detector antibody is purified anti-SorCS2 F7100, however the sensitivity is not high.
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0006 by Lars Meinertz Byg
does not IP murine sorCS2 to a considerable extent
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0006 by Lars Meinertz Byg
Great for spotting two-chain sorcs2
I used it in a 1 ug / IP dilution in stabily SorCS2-transfected SY5Y-cells.
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0006 by Ann-Kathrin Reuschlein
Used in RN22 cells transfected with human SorCS2 variants.
2 ug per IP.
Binds better to two-chain SorCS2, than one-chain SorCS2.
Okay IP.
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0006 by Simon Glerup
Excellent for staining of human SorCS2 expressed in HEK293 cells.
Dilution 1 ug/ml
Also great detection antibody in sandwich ELISA for shedded human SorCS2.
Dilution 1 ug/ml
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0006 by Anja A, Aarhus
Mouse monoclonal antibody generated in the lab of Anders Nykjær, Aarhus University.
Works in immunofluorescense using PFA-fixed primary mouse hippocampal neurons. 10 ug/ml
Knockout neurons were used as negative control.
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