SorCS2, Academia, Academia-0004
What do reviewers generally think about Academia-0004 from Academia?
Researchers report SorCS2, Academia, Academia-0004 is specific but low-sensitivity in ELISA. One reviewer points out that it gives a specific but low signal as a 1 µg/mL detector with rabbit anti-SorCS2 F7100 capture, while another finds it not very sensitive as a 10 µg/mL capture, though it may work with purified F7100 detector. It works in Western blotting at 5 µg/mL and immunofluorescence at 10 µg/mL in PFA-fixed mouse hippocampal neurons, using knockout neurons as negative control. One reviewer notes diluted antibody can be frozen and reused for Western blotting.
User Reviews (4)
Review of: SorCS2, Academia, Academia-0004 by Sune Skeldal, Aarhus University
This antibody gives a specific but low signal as detector antibody in ELISA (1 µg/mL) detecting soluble purified SorCS2 in combination with rabbit anti-human SorCS2 extracellular domain (F7100) as capture antibody.
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0 not usefulReview of: SorCS2, Academia, Academia-0004 by Sune Skeldal, Aarhus University
In my hands not very sensitive antibody as capture antibody (10 µg/mL) in ELISA detecting purified soluble SorCS2. May be used when detector antibody is purified anti-SorCS2 F7100.
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0004 by Anja A, Aarhus
Diluted antibody can be frozen and reused several times for Western blotting
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0 useful 0 not usefulReview of: SorCS2, Academia, Academia-0004 by Simon Glerup, Aarhus
Mouse monoclonal antibody generated in the lab of Anders Nykjær, Aarhus University.
Works in immunofluorescense using PFA-fixed primary mouse hippocampal neurons. 10 ug/ml
Knockout neurons were used as negative control.
Also works in Western blotting. 5 ug/ml
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