SorCS1, Abcam, ab237618
What do reviewers generally think about ab237618 from Abcam?
Researchers generally find SorCS1 Abcam ab237618 useful for detecting overexpressed human or murine SorCS1 in CHO cells by Western blot and for immunoprecipitation of human SorCS1. Specificity is noted, but performance on endogenous samples is poor: it did not detect p0 or adult mouse brain SorCS1, and staining of mouse primary hippocampal neurons was not convincing. One reviewer points out that background is high in untransfected cells at a 1:100 concentration.
Information about this antibody
Vendor notes
User Reviews (4)
Review of: SorCS1, Abcam, ab237618 by Peter Lund Ovesen
Works well in Western blots detecting SorCS1 from CHO-cells overexpressing either murine or human SorCS1.
Does NOT detect endogenous SorCS1 from p0 and adult mouse brains. A sorcs1-/- brain lysate was used as neg. reference. Cell expressing sorCS1 was used as positive ref.
WB 1:500
0 useful
0 not usefulReview of: SorCS1, Abcam, ab237618 by Jenny BF
Rabbit polyclonal anti-sol-SorCS1 custom made at Dako, F7050, 5420.
This antibody was used for staining of CHO-cells stable expressing mouse SorCS1 variant c. It stains specific, but the background is also high in untransfected cells at 1:100 concentration.
Comments
0 useful 0 not usefulReview of: SorCS1, Abcam, ab237618 by Simon Glerup, Århus
Works nicely for IP of human SorCS1 expressed in CHO cells
Comments
0 useful 0 not usefulReview of: SorCS1, Abcam, ab237618 by Anja A, Aarhus
Custommade antibody raised against the extracellular domain of SorCS1.
We used 5 ug/ml for staining of mouse primary hippocampal neurons.
Not convincing.
Comments
0 useful 0 not useful
Comments