PSD95, Thermo Fischer Scientific, MA1-046
What do reviewers generally think about MA1-046 from Thermo Fisher Scientific?
Researchers report mixed results for PSD95 MA1-046. One reviewer points out that it worked for immunostaining post synaptic densities in primary hippocampal neurons at 1:100. However, two reviewers found it ineffective for immunostaining mouse hippocampal neurons and hippocampal synaptosomes, with no visible PSD95 clusters at 1:500 IF or 1:1000 WB, and one ranked it worst among tested PSD95 antibodies.
Information about this antibody
Vendor notes
User Reviews (3)
Review of: PSD95, Thermo Fischer Scientific, MA1-046 by Takahito Higashi, The University of Tokyo
This antibody works with immunostaining for identyfing post synaptic densities in a primary hippocampal neuron in a dillution 1:100.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
0 useful
0 not usefulReview of: PSD95, Thermo Fischer Scientific, MA1-046 by Anders Dalby
Did not work for immunostaining mouse hippocampal neurons. Clusters of PSD95 was not visible.
Neurons were fixed for 20 min in 4% PFA and permeabilized using PBS + 0.1% Triton X-100. After blocking, the neurons were incubated with antibody in a 1:500 dilution in blocking buffer.
This antibody was tested simultaneously with other antibodies for PSD95 (see below) and proved to be the worst.
PSD95 124 014 (Synaptic Systems) – Guniea pig
PSD95 p246 (Sigma) – Mouse (BEST)
PSD95 MAB 1596 (Chemicon/Millipore) – Mouse
Comments
0 useful 0 not usefulReview of: PSD95, Thermo Fischer Scientific, MA1-046 by Simon Glerup, Aarhus
Does not work in our hands. We have tested it on synaptosomal preparations from the hippocampus and for staining of primary mouse hippocampal neurons.
IF dilution 1:50
WB 1:1000
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