MAPK, Cell Signaling Technology, 9101

We have used this antibody to study BDNF and pharmacological induced phosphorylation of MAPK in primary mouse hippocampal neurons.

This antibody was used for immunoblotting of RIPA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000

Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716

This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines.
Dilution: 1:1000

This anitbody was tested on lysates from hippocampal neurons either stimulated with BDNF or similar volume of saline added to growth medium. The cells were stimulated for 10 minutes at 37 degrees. lysis buffer containing phosstop and protease inhibitor.

50ug protein was loaded per lane and the antibody was used at a concentration of 1:1000.

This antibody works very well for detecting pMAPK!

I have used this antibody for detection of phospho-MAPK in lysates (50 ug total protein) of unstimulated and NT3-stimulated starved rat Schwann cells. It works great as I only see bands in the stimulated cells. Dilution 1:1000 of stock.

This antibody worked very well for detecting differences in ERK 1/2 phosphorylation following stiumulation with BDNF of Sorcs2/trkB transfected SY5Y-cell lines. There was however also faint bands in the nonstimulated cells.
1:1000 dilution

I have used this antibody for IHC on Insulin-stimulated HEK293 cells as well as BDNF-stimulated hippocampal neurons. Works great! Showed high signal intensity and almost no background in the negative control. Dilution 1:250.

I have used this antibody in stimulated murine primary macrophages and 293 cells. It works great! Dilution 1:1000

I have used this antibody for detection of phospho-MAPK in stimulated murine primary macrophages. I have used 1:1000 of stock in WB.

This antibody is excellent for WB. We stimulated HEK cells transfected with human TrkB with increasing concentrations of BDNF for 10 min. Cells were lysed in buffer containing protease and phosphatase inhibitors. We observed a dose-dependent response to BDNF using this antibody. Two intense bands around 45 kDa were induced by BDNF.
WB dilution 1:1000

I have also tested this antibody on cultured hippocampal neurons stimulated with 1 nM of BDNF for 10 minutes with great succes. This works very well for detecting phosphorylation of MAPK.

This antibody was tested on 293 cells in culture. The cells were stimulated with either 0, 1, 10 or 100nM of insulin before being fixed and stained. This antibody showed clear difference in signal between 0 and 10 nM. This antibody works very well for detecting phosphorylation of Mapk.
Dilution 1:1000

Works great in Western blotting.

We have used it for detecting MAPK phosphorylation upon stimulation of SY5Y cells with GDNF.

WB dilution 1:1000
Diluted antibody can be frozen and reused.