Neurofilament 200, Sigma-Aldrich, N4142

I have used this antibody for immunocytochemistry of primary hippocampal neurons in a dilution of 1:250. It worked great.

This antibody works with immunostaining for identyfing axons in a primary hippocampal neuron in a dillution 1:1000. The NF200 staining image mainly overlapped other axomal markers (ex. anti-tau1), but not completely.

For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).

Great marker! We used it for mouse hippocampal neurons and for cryosections of mouse brain. Dilution 1:80