Neurofilament 200, Sigma-Aldrich, N4142
What do reviewers generally think about N4142 from Sigma-Aldrich?
Researchers report Neurofilament 200, Sigma-Aldrich N4142, works well for immunocytochemistry and immunostaining of primary hippocampal neurons and mouse brain cryosections, using dilutions of 1:80, 1:250, and 1:1000. It is described as a great marker for identifying axons. One reviewer points out that NF200 staining mainly overlapped other axonal markers such as anti-tau1, but not completely. No major complaints are mentioned.
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User Reviews (3)
Review of: Neurofilament 200, Sigma-Aldrich, N4142 by Yutaro Kashiwagi, The University of Tokyo
I have used this antibody for immunocytochemistry of primary hippocampal neurons in a dilution of 1:250. It worked great.
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0 not usefulReview of: Neurofilament 200, Sigma-Aldrich, N4142 by Takahito Higashi, The University of Tokyo
This antibody works with immunostaining for identyfing axons in a primary hippocampal neuron in a dillution 1:1000. The NF200 staining image mainly overlapped other axomal markers (ex. anti-tau1), but not completely.
For more information see Higashi et al., Synapse Elimination Triggered by BMP4 Exocytosis and Presynaptic BMP Receptor Activation, Cell Reports, vol 22, issue 4, p919-929, January 23, 2018 (https://doi.org/10.1016/j.celrep.2017.12.101).
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0 useful 0 not usefulReview of: Neurofilament 200, Sigma-Aldrich, N4142 by Simon Glerup, Aarhus
Great marker! We used it for mouse hippocampal neurons and for cryosections of mouse brain. Dilution 1:80
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