MAP kinase, Cell Signaling Technology, 4695
What do reviewers generally think about 4695 from Cell Signaling Technology?
Researchers generally rate this MAP kinase antibody favorably for Western blotting, typically at 1:1000, with one user using 1:2000. It is described as specific, showing two bands around 42 and 44 kDa in rat primary Schwann cell lysates, and works in murine macrophages, 293 cells, and mouse spinal cord tissue. One reviewer points out that the antibody is against MAPK and not phospho-MAPK. Ratings are strong for immunofluorescence and good for IHC, though one user advises using the antibody only once.
Information about this antibody
Vendor notes
User Reviews (5)
Review of: MAP kinase, Cell Signaling Technology, 4695 by Margie Sutton, University of Texas MD Anderson Cancer Center
WB dilution 1:2000
IF rating: 5 stars
IHC rating: 4 stars
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
0 useful
0 not usefulReview of: MAP kinase, Cell Signaling Technology, 4695 by Maj Ulrichsen
This antibody detects MAKP in rat primary Schwann cell lysates(50 ug). Dilution 1:1000. It appears to be very specific for MAPK, as I only see two lanes (app. 42 kDa and 44 kDa) in my blot.
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0 useful 0 not usefulReview of: MAP kinase, Cell Signaling Technology, 4695 by Jakob Vejby Larsen, Aarhus University
I have used this antibody in murine primary macrophages and 293 cells. It is very good! Dilution 1:1000
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0 useful 0 not usefulReview of: MAP kinase, Cell Signaling Technology, 4695 by Mads Fuglsang Kjolby
WB 1:1000 in primary murine macrophages.
OBS: This antibody is against MAPK and NOT phospho MAPK! (Cell Signaling 4695)
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0 useful 0 not usefulReview of: MAP kinase, Cell Signaling Technology, 4695 by Mette R
WB dilution 1:1000 on mouse spinal cord tissue
100 ug per lane
Use only once!
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0 useful 0 not useful
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