LAMP-1, Abcam, AB25630
What do reviewers generally think about AB25630 from Abcam?
Researchers report that LAMP-1, Abcam, AB25630 works well for lysosomal immunostaining in human-derived cells, including HEK293 and HepG2, typically at 1:200 dilution after 0.1% Triton X-100 permeabilization and 2% BSA blocking. One reviewer points out that the antibody stops working if stored at 4C and should be aliquoted at -20C. Another notes saponin may give better lysosome staining than Triton X-100, and recommends leupeptin/pepstatin treatment before fixation for improved visualization.
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User Reviews (3)
Review of: LAMP-1, Abcam, AB25630 by
This antibody was used for immunostaining HEK293 cells. Lysosomes were evenly stained througout the cells. Prior staining the cells were permebealized with 0.1% Triton X-100 and blocked with 2% BSA.
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0 not usefulReview of: LAMP-1, Abcam, AB25630 by Julie Torvund-Jensen
This antbibody works nicely for immunostaining of lysosomes in HEK293 and HepG2 cell.
In my experience, permeabilization with Triton X-100 (0.1 %) works fine.
Antibody dilution: 1:200
NB! Note that this antibody stops working if stored at 4C. Should be stored in small aliquots at -20C.
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0 useful 0 not usefulReview of: LAMP-1, Abcam, AB25630 by Simon Glerup, Aarhus
We use this antibody as a lysosomal marker in human-derived cell lines, e.g. 293 cells.
Dilution 1:200.
The image above shows human GDNF (green) bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1. Cells are also stained for Lamp-1 (red) using mAb H4A3.
NB. For mouse cell lines, we use Abcam 24170.
It is important to consider what detergent is used for permeabilization of cells. Saponin appears to give much better lysosome stainings than Triton X-100.
For inhibition of lysosomal degradation and better visualization of lysosomes, incubate with 50 ug/ml leupeptin and pepstatin for 24 h before fixing the cells. During this period, the leupeptin/pepstatin medium has to be changed every six hours.
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