Insulin receptor, Santa Cruz Biotechnology, sc711
What do reviewers generally think about sc711 from Santa Cruz Biotechnology?
Researchers report sc711 works for Western blotting at 1:1000 in 293 cells and human/murine tissues, and for IP, with one reviewer noting it works very well for precipitating transfected human receptor using 1 µg per 75 µl beads. It also gives punctate IF in primary myocytes and murine cells at 1:200 and believable staining in hippocampal neurons at 1:250. One reviewer points out that lack of negative controls is a problem for IF interpretation.
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User Reviews (3)
Review of: Insulin receptor, Santa Cruz Biotechnology, sc711 by Christian Vægter
Used for IF on primary myocytes and murine cell lines 1:200. Gives nice punctate staining (but lack of negative controls is a problem).
0 useful
0 not usefulReview of: Insulin receptor, Santa Cruz Biotechnology, sc711 by Mads Kjolby
I have used this antibody for precipitation (IP) of the human insulin receptor tranfected into HEK293 cells. Works very good. I coated the the beads with 1 ug/75 ul beads = 1 IP.
It also works very well in western blots for detection of the insulin receptor and the proform in both human tissue and murine tissue. Dilution 1:1000.
I have also used it for immunostaing in hippocampal neurons (1:250), and it gives a believable staining.
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0 useful 0 not usefulReview of: Insulin receptor, Santa Cruz Biotechnology, sc-711 by Simon Glerup, Aarhus
The antibody recognizes endogenous insulin receptor in 293 cells in Western blotting 1:1000 dilution.
Works reasonable for IP.
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