His, Genscript, A00186
What do reviewers generally think about A00186 from Genscript?
Researchers generally report that His, Genscript, A00186 performs well for detecting His-tagged proteins by western blotting and for IP/co-IP, including His-tagged PCSK9 in CHO or HEK293 media, with dilutions of 1:1000 to 1:5000 in milk buffer. Several note the diluted antibody can be stored frozen and reused multiple times. One reviewer points out that the antibody gives unspecific nuclear staining in immunofluorescence, while another notes strong background from a pre-stained protein marker. Overall, it is considered reliable for blotting and IP but may need optimization for IF.
Information about this antibody
Vendor notes
User Reviews (5)
Review of: His, Genscript, A00186 by Heidi Gytz Olesen, McGill University, Canada
THE His Tag Antibody, Genscript A00186-SZ
Short protocol:
1:5000 dilution in 5% low fat milk powder in PBS-T and incubate overnight at 4°C.
Dynabeads protein A kits (Invitrogen) were used for IP. Longer incubation of the antibody in the Ab binding and washing buffer of 1 h was used for optimal binding.
Review:
Worked perfectly for both immunoblotting and co-IP. The diluted antibody could be stored at -20°C after use and be reused 5-10 times for immunoblotting.
0 useful
0 not usefulReview of: His, Genscript, A00186 by Sune Skeldal, Aarhus University
Good antibody against His-tag for western blotting. Detected his-tagged constructs expressed in media from CHO cells with good result. I used 1:2500 dilution.
Comments
0 useful 0 not usefulReview of: His, Genscript, A00186 by Camilla Gustafsen, Aarhus University
This antibody (1:2500) specifically recognizes 6His tagged PCSK9 secreted to the media of stably transfected HEK293 cells. No band is detected in the lane loaded with media from cells transfected with un-tagged PCSK9. However, a strong background signal is detected from the SeeBlue Plus2 pre-stained protein marker. See inserted figure
Comments
0 useful 0 not usefulReview of: His, Genscript, A00186 by Camilla Gustafsen, Aarhus University
We have used this antibody for WB detection of His tagged PCSK9 produced in CHO cells.
Great result. No background signal
We dilute 1:2500 in 5 % milk buffer and reuse several times
Comments
0 useful 0 not usefulReview of: His, Genscript, A00186 by Simon Glerup, Aarhus
Reasonable for detecting His-tagged proteins by Western blotting.
WB dilution 1:1000.
Diluted antibody can be frozen and reused several times.
In IF, the antibody gives unspecific staining of the nucleus. Not cool!
Good for IP of His-tagged proteins.
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0 useful 0 not useful
Comments