Flotilin, Abcam, ab40753
What do reviewers generally think about ab40753 from Abcam?
Researchers generally report that Flotilin, Abcam, ab40753 performs well in Western blot and immunofluorescence, detecting a specific 48 kDa band in 293 and SY5Y lysates and brain homogenates, and plasma membrane clusters in retinoic acid differentiated SY5Y cells. It is used as a lipid raft marker after sucrose gradient fractionation, with WB dilution 1:1000 and IF dilution 1:100. One reviewer points out that it does not work at all for reprobing older membranes, although another says it can reprobe stripped membranes.
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User Reviews (2)
Review of: Flotilin, Abcam, ab40753 by Anja A, Aarhus
Does not work at all for reprobing of older membranes..
0 useful
0 not usefulReview of: Flotilin, Abcam, ab40753 by Simon Glerup, Aarhus
Great antibody. Detects a band in WB of the expected size – 48 kDa. No other bands are detected. We have used it on 293 and SY5Y cell lysates and brain homogenates as a marker for lipid rafts after sucrose gradient fractionation experiment.
The antibody can be used reprobing of stripped membranes.
WB dilution 1:1000.
We have also used it for staining retinoic acid differentiated SY5Y cells. Detects clusters associated with the plasma membrane.
IF dilution 1:100
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