CLN3, Abcam, 75959
What do reviewers generally think about 75959 from Abcam?
Researchers report that CLN3, Abcam, 75959 performs well for immunoprecipitation of endogenous CLN3 from HEK293 cells and mouse primary astrocytes using 0.7 µg per IP, and for Western blotting at 1:1000, recognizing a specific ~60 kDa band in HEK293, astrocyte, and hippocampal neuron lysates. It also gives strong immunofluorescence at 1:100 in these cells, showing intracellular vesicular and tubular localization. One reviewer points out that the antibody recognized the expected 60 kDa protein specifically across multiple cell types.
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User Reviews (3)
Review of: CLN3, Abcam, 75959 by Camilla Gustafsen, Aarhus University
anti-CLN3 ab75959
We have also used the antibody for IP (0.7µg/IP) of endogenous CLN3 from a primary culture of mouse astrocytes.
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0 not usefulReview of: CLN3, Abcam, 75959 by Camilla Gustafsen, Aarhus University
anti-CLN3 ab75959
We have used this antibody for IP of endogenous CLN3 from HEK293 cells. We used 0.7 µg per IP with good results
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0 useful 0 not usefulReview of: CLN3, Abcam, 75959 by Anja A, Aarhus
Great antibody! Recognized specifically a protein in Western blotting around 60 kDa (the expected size) in lysates from a number of cell types including HEK293 cells, mouse primary astrocytes and hippocampal neurons.
Strong immunofluorescense staining of HEK293, astrocytes and hippocampal neurons. CLN3 appears to be localized in intracellular vesicular and tubular structures.
WB 1:1000
IF 1:100
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