Caspase-3, Cell Signaling Technology, 9661
What do reviewers generally think about 9661 from Cell Signaling Technology?
Researchers generally report that Caspase-3, Cell Signaling Technology, 9661 performs well for ICC, IHC, immunofluorescence, and Western blot, detecting cleaved or activated Caspase-3 in imaginal discs, brain, VNC, mouse muscle, neuronal lysates, tumor xenografts, and injured nerve/DRG at 1:100 to 1:1000. One reviewer points out that a batch may show cross-reactivity with uncleaved Caspase-3, and another detected only pro-Caspase-3, not cleaved active Caspase-3, in primary CD4+ T cells, recommending skim milk blocking.
Information about this antibody
Vendor notes
User Reviews (6)
Review of: Caspase-3, Cell Signaling Technology, 9661 by Bing Zhang initiative
Great for ICC on imaginal discs, brain and VNC. Dilution: 1:100
0 useful
0 not usefulReview of: Caspase-3, Cell Signaling Technology, 9661 by Ana Jovicic, Genentech
IHC was performed on fixed frozen mouse muscle tissue. The antibody worked great.
Dilution used: 1:200
For more information see Yartseva et al. Cell Reports 2020, “ Heterogeneity of Satellite Cells Implicates DELTA1/NOTCH2 Signaling in Self-Renewal”.
https://www.sciencedirect.com/science/article/pii/S2211124719317656
Comments
0 useful 0 not usefulReview of: Caspase-3, Cell Signaling Technology, 9661 by Sérgio Almeida
This antibody has worked quite well for the detection of cleaved Caspase-3 in lysates of primary mouse neuronal cultures by WB (1:1000).
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0 useful 0 not usefulReview of: Caspase-3, Cell Signaling Technology, 9661 by Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University
This antibody was used for immunofluorescent staining of paraffin-embedded sections of PFSK-1 and D-283 tumor xenografts.
Dilution: 1:100 in PBS or in blocking/permeabilization buffer (buffer containing 5% normal goat serum (NGS) and 0.1-0.3% Triton-X 100 in 1xPBS)
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
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0 useful 0 not usefulReview of: Caspase-3, Cell Signaling Technology, 9661 by Sara Konstantin Nissen
Caspase-3 (8G10) Rabbit mAb #9665 Cell signaling
This AB is suppose to detect pro-caspase3 as well as celaved caspase-3. However, I only maneged to detect pro-caspase3 in primary blood purified CD4+ T cells ( treated with stausporine). One fine band for the pro-caspase3, but no band for the celeaved active capse3, therefor only 2 stars. Remeber to block in skim milk.
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0 useful 0 not usefulReview of: Caspase-3, Cell Signaling Technology, 9661 by Simon Glerup, Aarhus
We have used this antibody in immunofluorescence on sections of sciatic nerve and dorsal root ganglions following injury.
There is a nice increase in activated caspase-3 staining in the DRG of the injured side. And also in the fiber in Schwann cells closed to the site of injury. This increase is not seen in the uninjured side.
The antibody only gets three stars because there appears to be some batch variations for this antibody. One of the batches we have acquired may show cross reactivity with uncleaved caspase-3.
Dilution 1:200
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0 useful 0 not useful
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