Calnexin, Abcam, ab22595
What do reviewers generally think about ab22595 from Abcam?
Researchers report Calnexin Abcam ab22595 works reasonably well for immunocytochemistry in HEK293, HepG2, and primary hepatocytes, with dilutions of 1:20, 1:250, or 5 ug/ml after PFA fixation and Triton-X 100 permeabilization. It is also used for Western blotting of mouse liver homogenates as an ER marker after subcellular fractionation, with no background bands at 1:1000 or higher dilution. One reviewer points out that a background control was obviously not available in their immunocytochemistry experiment.
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User Reviews (4)
Review of: Calnexin, Abcam, ab22595 by Sune Skeldal, Aarhus University
Detects human calnexin in HEK293 cells in immunocytochemistry reasonable well using a dilution of 1:20. However a background control is obviously not available here.
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0 not usefulReview of: Calnexin, Abcam, ab22595 by Mads Fuglsang Kjolby, Mayo Clinic
HepG2 cells and/or primary hepatocytes were fixed in 4% PFA, permeabilized (0.1% Triton-X 100), blocked in 10% FCS in PBS, and incubated with anti-Calnexin 1:250 (ab22595, Abcam, Cambridge, England) for 24 hrs at 4°C.
In Kjolby et al. Cell Metab, Suppl material, Figure S3
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0 useful 0 not usefulReview of: Calnexin, Abcam, ab22595 by Simon Glerup, Aarhus University
This antibody seems to work OK for staining of calnexin i HepG2 cells.
Cells were fixed in 4% PFA for 20 min at RT.
Washing was carried out using PBS containing 0.1% Triton X100.
Dilution 5 ug/ml.
See image above. Calnexin (red). Cells are also stained for sortilin (green, mAb F11) and nuclei using Hoechst (blue).
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0 useful 0 not usefulReview of: Calnexin, Abcam, ab22595 by Camilla Gustafsen
This antibody can be used for WB of mouse liver homogenates. We used it as ER marker after subcellular fraktionation. No background bands. Use 1:1000 or dilute more
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