Beta-galactosidase, MP Biomedicals, 56033
What do reviewers generally think about 56033 from MP Biomedicals?
Researchers consistently report that MP Biomedicals 56033 anti-beta-galactosidase performs well for western blotting of LacZ-expressing viruses in cell or tissue lysates, including in vivo and in vitro systems. It is described as the best beta-galactosidase antibody they have used, with successful detection on Tris or bisTris gels transferred to PVDF and blocked in milk. One reviewer points out that they use it at 1:2000 with HRP secondary antibody and ECL, while others report 1:1000. No complaints are mentioned.
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User Reviews (3)
Review of: Beta-galactosidase, MP Biomedicals, 56033 by Mads Kjolby, Aarhus University
I have used viruses expressing LacZ (beta-galactosidase) in vivo and in vitro (mice and cells). The antibody anti-beta-galactosidase from MP Bio (56033) is the best I have used for western blotting. I use it on lysates (TNE+complete+phosstop) run on home-made 4-16% tris gels, or NuPage 4-12% bisTris gels, transfer to PVDF membranes, and block in milk. I use the antibody at 1:2000 and develop with HRP 2nd abs and ECL.
0 useful
0 not usefulReview of: Beta-galactosidase, MP Biomedicals, 56033 by Mads Kjolby
I have used the ab 56033 from MP Bio against beta-galactosidase on tissue or cell lysates subjected to viral vectors with LacZ (beta-galactosidase). It is the best antibody I have used (see Kjolby et al., Cell Metab september 2010).
In WB: 1:1000 dilution.
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0 useful 0 not usefulReview of: Beta-galactosidase, MP Biomedicals, 56033 by Camilla Gustafsen
very good antibody for WB (1:1000)
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0 useful 0 not useful
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