Beta-actin, Sigma-Aldrich, A5441

We have used this antibody to detect beta-actin in primary mouse hippocampal neurons and homogenates of mouse hippocampus.

Works perfectly for western blot – both for human, murine and rat cell lysates.
Can be reused at least 5 times

Great antibody for WB on cell lysates.

For more information see Nourreddine et al. Cell Reports 2020, “NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay”.
https://www.sciencedirect.com/science/article/pii/S2211124720306136#

This antibody worked great for western blotting.

For more information see Zaborowski et al. Cell Reports 2019, Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles
https://www.cell.com/cell-reports/fulltext/S2211-1247(19)30310-9#secsectitle0025

This antibody works great to visualize the cytoskeleton of HEK293 cells. Dilution 1:1000.

Very good antibody that gives a strong signal.
Dilution 1:5000

This antibody was used as Western blot loading control for lysates of human CNS embryonal tumors, medulloblastomas and tumor cell lines D283, D324 and PFSK-1.
Dilution: 1:5000 (1x TBST with 5% w/v BSA)

Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716

Works very well for loadning control for western blot on lysates from mouse striatum and cortex. Dilution 1:5000.

A excellent antibody for Western blots. I have diluted this antibody 1:10.000 and still get a very high, specific signal from HepG2 cells. The antibody solution can be reused several times.

I have used this antibody as af loading control for mouse liver and adrenal gland homogenates (20-60 µg total protein). I dilute 1:5000 and reuse the antibody several times

This antibody works well in western blotting using 1:3000 dilution.

I used this as a primary antibody for western blotting of cultured hippocampal neurons at a dilution of 1:5000 with excellent results.

I routinely use this antibody as a western blotting loading control in a dilution of 1:5000. It gives a very strong band when using 10 ug cell lysate. I have tried it on rat cell cultures and human cell cultures as well as mouse primary neurons.

I tried this antibody in a 1:1000 dilution in immunoprecipitation of Human b-actin (SY5Y-cells), with poor results. however, it works great for westernblotting in a 1:3000 solution, and can easily be reused.

i have used this for wb loading control from mouse hippocampal tissue lysates. Works excellent for detection.

used at a dilution of 1:5000. Can be frozen and reused.

We have used this antibody (1:2500) for WB loading control (HepG2 lysate).

Great antibody in Western blotting. Very high signal. In our Western blotting imaging system, we get a strong distinct within seconds.

Works also on stripped membranes. The diluted used antibody can also be frozen and used again.