Phospho-Akt, Cell Signaling Technology, 9271
What do reviewers generally think about 9271 from Cell Signaling Technology?
Researchers generally report that Phospho-Akt CST 9271 is reliable for western blotting, producing a specific 60 kDa band in primary neurons, neuroblastoma, lung cancer, fibroblast, and kidney tissue lysates. Typical dilutions are 1:500 to 1:2000, often in 5% BSA/TBS/Tween with overnight 4C incubation. One reviewer points out that 5% BSA is optimal and the antibody can be reused twice. It is also rated well for immunofluorescence and IHC, with no complaints noted.
Information about this antibody
Vendor notes
User Reviews (5)
Review of: Phospho-Akt, Cell Signaling Technology, 9271 by Margie Sutton, University of Texas MD Anderson Cancer Center
WB dilution 1:2000
IF rating: 5 stars
IHC rating: 4 stars
For more information see Sutton et al. Cell Reports 2019, “DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters”
https://www.sciencedirect.com/science/article/pii/S221112471931527X#sec5
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0 not usefulReview of: Phospho-Akt, Cell Signaling Technology, 9271 by Argyris Spyrou, Department of Immunology, Genetics and Pathology, Uppsala University
This antibody was used for immunoblotting of RIBA buffer cell lysates obtained from tumor cell lines PFSK-1 and D324.
Dilution: 1:1000
Ref: Spyrou et al., Mol Cancer Ther, 2017 Aug; 16(8): 1705-1716
Comments
0 useful 0 not usefulReview of: Phospho-Akt, Cell Signaling Technology, 9271 by Christina Demuth
This antibody is used for evaluating activity of the EGF-receptor pathway after stimulation or inhibition of EGFR in lung cancer cell lines. The antibody is very reliable. We have found that dilution in 5 % BSA is optimal.
Dilution: 1:500
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0 useful 0 not usefulReview of: Phospho-Akt, Cell Signaling Technology, 9271 by Anne Mayntzhusen (TVS group)
Phospho-Akt (Ser473) rabbit antibody #9271 from Cell Signaling gives one specific band at 60 kDa.
I have used the antibody for western blotting with 20 ug WCE of cultured primary mouse hippocampal neurons and with 20 ug WCE of the human neuroblastoma cell line SH-SY5Y in the dilution 1:1000 and incubation at +4C ON.
The antbody can be reused 2 times.
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0 useful 0 not usefulReview of: Phospho-Akt, Cell Signaling Technology, 9271 by Maria Lume
rabbit Phospho-Akt (cat no 9271) from Cell Signaling works very nicely in WB. Have detected pAKT levels from both cell-lines (MG87 fibroblasts) as well as from embryonic kidney tissue lysates (E14).
Used dilution 1:1000, 5%BSA/TBS/Tween, incubates O/N at +4C
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