GFAP, Agilent Dako, Z0334

Great antibody for IF. Concentration indicated by manufacturer’s protocol.

For more information see Micali et al. Cell Reports 2020, “Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates”.
https://www.sciencedirect.com/science/article/pii/S2211124720305489

A preferred tool for the demonstration of many (but not all) astrocytes is rabbit-anti-GFAP from Dakocytomation (Glostrup, Denmark, Z0334). This immunoreagent raised against the cow antigen found to be an excellent marker for astrocytes and astrogliosis in human tissues by Halliday et al 1996 Neurosci Lett. Rabbit-anti-GFAP was very useful to reveal active astroglia for instance after traumatic brain injury or stroke in rodents (Härtig et al 2009 – Figs 1-5). Notably, this antibody detects astroglia in various animal species and recently also in tenrecs (Mack et al 2018 Brain Struct Funct 2018 – Figs 1 and 2).

Halliday et al. 1996 Neurosci Lett; https://www.sciencedirect.com/science/article/abs/pii/0304394096125921?via%3Dihub
Härtig et al. 2009 Journal of Chemical Neuroanatomy: https://www.sciencedirect.com/science/article/pii/S0891061808001257?via%3Dihub
Mack et al. 2018 Brain Struct Funct: https://link.springer.com/article/10.1007%2Fs00429-018-1730-1

Used for Immunofluorescence of transfected RN22 cells treated with 4% PFA in PBS for 5 min. Washed 10 min in PBS. And penetrated with 0.5 % Triton x-100 in PBS for 7 min at 4 C.
Best dilution was 1:500.
Good beautifully filament staining.

Great antibody for immunocytochemistry and immunohistochemistry on mouse tissues.

It beautifully stains formaldehyde-fixed mouse primary astrocytes and astrocytes in cryosections of mouse brain.

We recommend 1:500 up to 1:5000 dilution of the antibody.

The image above shows a primary mouse cortical astrocyte stained for GFAP (red) and the receptor SorLA (green, SorLA mAb 20C11).