Phospho-Ret (Y905), R&D Systems, AF3269
Information about this antibody
Target
Alt. Targets
Vendor
Catalog No
AF3269
Application
Clonality
pAb
Origin
Rabbit
Reactivity
Human
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (1)
Review of: Phospho-Ret (Y905), R&D Systems, AF3269 by Simon Glerup, Aarhus
The Ab doesn´t work for Western blotting!
We have tried this phospho-Ret antibody in cells transfected with human Ret stimulated with various GDNF concentrations.
We checked that GDNF-induced Ret phosphorylation indeed took place by IP of Ret and then WB using anti-pTyr.
We also tried neuroblastoma cells expressing endogenous Ret with no success.
The concentration recommended by R&D Systems (0.5 ug/ml) was used in both cases.
0 useful
0 not useful
Comments