Neuropilin-2, R&D Systems, AF567
What do reviewers generally think about AF567 from R&D Systems?
Researchers report that Neuropilin-2, R&D Systems, AF567 works well in Western blotting and immunofluorescence. It gave the best signal in mouse primary hippocampal neurons and rat RN22 cells, recognized 1-2 bands at predicted molecular weights, and produced nice surface-localized staining on mouse hippocampal neurons and astrocytes. One reviewer points out that it gave excellent surface staining and apparent staining of dendritic spines on formaldehyde-fixed mouse hippocampal neurons at 2 ug/ml. Reported dilutions were 0.1 ug/ml for Western blot and 5 ug/ml for immunostaining.
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User Reviews (2)
Review of: Neuropilin-2, R&D Systems, AF567 by Simon Glerup, Aarhus
Just tested anti-neuropilin-2 together with anti-neuropilin-1 on mouse hippocampal neurons. P0. 14 DIV. Formaldehyde fixed.
Dilution 2 ug/ml.
Excellent surface staining and staining of what appears to be dendritic spines.
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0 not usefulReview of: Neuropilin-2, R&D Systems, AF567 by Kathrin Weyer, Aarhus
We used anti-neuropilin-2 for Western blots on a number of human cell lines and mouse primary cultures.
We obtained the best signal in mouse primary hippocampal neurons and in rat RN22 cells. The antibody appears highly specific and only recognized 1-2 bands of the predicted molecular weight of full lenght neuropilin-2 and a truncated splice variant.
The antibody was also used for immunofluorescense on mouse hippocampal neurons and astrocytes. Staining was nice and surface localized.
Generally, expression pattern and subcellular localization appeared to be similar to what was observed using anti-neuropilin-1 from R&D.
Western blot concentration: 0.1 ug/ml
Immunostaining: 5 ug/ml
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