Neuropilin-1, R&D Systems, AF566
Information about this antibody
Target
Alt. Targets
Neuropilin-1
Vendor
Catalog No
AF566
Application
Clonality
pAb
Origin
Goat
Reactivity
Mouse, Rat
Conjugation
N/A
Product Link
N/A
Vendor notes
No vendor notes available for this antibody.
User Reviews (2)
Review of: Neuropilin-1, R&D Systems, AF566 by Simon Glerup, Aarhus
I have just tested it on primary mouse hippocampal neurons. PFA fixed.
It works very nice. Beautiful surface staining of soma and neurites.
IF dilution 2 ug/ml
Review of: Neuropilin-1, R&D Systems, AF566 by Kathrin Weyer, Aarhus
We used anti-neuropilin for Western blots on a number of human cell lines and mouse primary cultures.
We obtained the best signal in mouse primary hippocampal neurons and in rat RN22 cells. The antibody appears highly specific and only recognized 1-2 bands of the predicted molecular weight of full lenght neuropilin-1 and a truncated splice variant.
The antibody was also used for immunofluorescense on mouse hippocampal neurons and astrocytes. Staining was nice and surface localized.
Western blot concentration: 0.1 ug/ml
Immunostaining: 5 ug/ml
Comments