Neuropilin-1, R&D Systems, AF566
What do reviewers generally think about AF566 from R&D Systems?
Researchers report Neuropilin-1 AF566 works well in Western blot and immunofluorescence, giving specific surface staining of mouse hippocampal neurons and astrocytes and 1-2 bands at predicted sizes in primary cultures and cell lines. One reviewer points out that PFA-fixed primary mouse hippocampal neurons showed beautiful surface staining of soma and neurites at 2 ug/ml, while another notes best Western signal in mouse hippocampal neurons and rat RN22 cells at 0.1 ug/ml.
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User Reviews (2)
Review of: Neuropilin-1, R&D Systems, AF566 by Simon Glerup, Aarhus
I have just tested it on primary mouse hippocampal neurons. PFA fixed.
It works very nice. Beautiful surface staining of soma and neurites.
IF dilution 2 ug/ml
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0 not usefulReview of: Neuropilin-1, R&D Systems, AF566 by Kathrin Weyer, Aarhus
We used anti-neuropilin for Western blots on a number of human cell lines and mouse primary cultures.
We obtained the best signal in mouse primary hippocampal neurons and in rat RN22 cells. The antibody appears highly specific and only recognized 1-2 bands of the predicted molecular weight of full lenght neuropilin-1 and a truncated splice variant.
The antibody was also used for immunofluorescense on mouse hippocampal neurons and astrocytes. Staining was nice and surface localized.
Western blot concentration: 0.1 ug/ml
Immunostaining: 5 ug/ml
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