SorLA, Academia, Academia-0047

This mAb was generated in the lab of Professor Claus Munck Petersen, Aarhus University. It is commercially available from Sigma Aldrich/Merck as MABN1793.

I have used the SorLA antibody 20:C11 on cryosectioned, PFA fixated mouse dorsal root ganglia, on which it works fine.
I have used it in combination with Fab buffer (Donkey anti-mouse)

Dilution: 1:500

It works very well for immunostaining of PFA fixed cells at a dilution of 1:100

I have used this antibody for immunostainings on adult mouse barin tissue. It worked very well for detection of SorLA.

concentration 10ug/ml

I have used this antibody for immunostainings on adult mouse barin tissue as well as cultured hipoocampal neurons. In both cases this antibody worked very well for detection of SorLA.

concentration 10ug/ml

We used this SorLA mAb for ELISA on human serum. Development takes about 20-30 minutes, gives a nice std curve and good consistent measurements for serum diluted 5, 10 and 20 times in 1 % BSA.

For best results we used Nunc MaxiSorp 96 well plates.

Concentration was 1 ug/ml

I have used the 20C11 antibody for staining of brain sections from cortex (nice neuron staining in vesicle strucures / TGN) and also in dopaminergic neurons in substantia nigra in mesencephalon (see Glerup et al., Cell reports, 2013) (double staining with anti-TH and SorLA).

OBS: In mice it is necessary to block with fab fragments if the tissue has to much background.

Not reactive against rat SorLA

This is a nice SorLA mAb produced in Claus M. Petersen lab, Aarhus. Cited in Klinger et al. Journal of Cell Science 2011

I have used it for Western blotting on brain from wt and SorLA knockouts. It detects a specific band around 250 kDa.

It is great for staining glia and neurons both in ICC and IHC. Knockouts are blank.

For WB 1 ug/ml
For ICC 10 ug/ml

The image above shows a primary mouse cortical astrocyte stained using SorLA mAb 20C11 (green) and GFAP (red, DAKO)