Rad51, Cell Signaling Technology, 8875

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Brunner et al. eLife, 2020. Brunner et al. validated this antibody for the detection of endogenous human RAD51 by Western blotting using control and RAD51-KO MDA-MB-231 cell lysates. Brunner et al. used this antibody for the detection of endogenous human RAD51. CRISPR/Cas9-mediated targeting of RAD51 was performed in MDA-MB-231 cells; however, the authors state that they were unable to generate stable RAD51-KO cell lines. Two independent RAD51-KO clones were analyzed. A clear RAD51 band was detected in the control lysate. No corresponding band was observed in KO clone #1, whereas a RAD51 band remained detectable in KO clone #2 (Figure 5—figure supplement 1). No full blot image was available. Based on the available data, we have assigned a four-star rating, but we highly encourage other scientists to share their observations.