ApoE, Meridian Bioscience, K74180B

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. APOE encoding apolipoprotein E (apoE) is the 5th most studied gene on the list. In Zhao et al., Acta Neuropathologica (2021), the authors investigate APOE in human iPSC-derived cerebral organoids. Meridian Bioscience K74180B is tested by WB (1:1000) using day-90 organoid lysates (parental control and isogenic APOE KO). Data are shown in Fig 1B and Supp Fig 6B–D. A band (37 kDa) is detected in controls but not in KO samples from two independent parental/KO models, supporting specific APOE detection. β-actin provides a loading control, and mRNA measurements support KO in the second model. Little background is seen within the displayed regions, but the blots are cropped, limiting assessment of additional nonspecific bands. We have rated K74180B based on the available information and according to the pAbmAbs criteria.