Sphingosine 1-phosphate receptor 1, Abcam, ab233386

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Sphingosine 1-phosphate receptor 1 (S1PR1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria. YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human S1PR1. The collection of antibodies was tested using SK-HEP-1 WT and S1PR1 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference). In WB (1:1000 ab dilution) ab233386 detected a double band of appr. 42 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. However, faint bands of both lower and higher molecular weight were observed in WT cell lysate. A smear ranging from 40-245 kDa was detected following IP (2 µg Ab used), this smear was markedly more intense compared to band in input extract (4% of starting material) and no band was detected in immunodepleted extract (4% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:400 ab dilution), clear staining was observed in WT cells and KO cells showed no stain.