Syntaxin-binding protein 1, Thermo Fisher Scientific, PA1-742

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Syntaxin-binding protein 1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 12 commercial research antibodies against human Syntaxin-binding protein 1 encoded by STXBP1.

The collection of antibodies was tested using U-87 MG WT and STXBP1 KO cells in the applications WB (WT and KO cell lysates/40 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) PA1-742 detected a band of appr. 67 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. An intense band of lower molecular weight was observed in both genotypes. No band was detected following IP (2 µg Ab used). In IF (1:1000 ab dilution), comparable staining intensity was observed in KO and WT cells, apart from one WT cell displaying pronounced staining