Ubiquitin-specific protease 30, Thermo Fisher Scientific, MA5-31401

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ubiquitin-specific protease 30 (USP30) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human Ubiquitin-specific protease 30 (USP30).

The collection of antibodies was tested using MCF-7 WT and USP30 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) MA5-31401 detected a band of appr. 58 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. An intense band of higher molecular weight was observed in both genotypes. A band was detected following IP (2 µl Ab used), this band was more intense compared to band in input extract (6% of starting material). A faint band was also detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does almost efficiently deplete the antigen from cell lysate. In IF (1:1000 ab dilution), comparable staining intensities was observed in KO and WT cells.