Ubiquitin-specific protease 30, Cell Signaling Technology, 57045

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ubiquitin-specific protease 30 (USP30) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human Ubiquitin-specific protease 30 (USP30).

The collection of antibodies was tested using MCF-7 and HAP1 (WT and USP30 KO cells) in the application WB (WT and KO cell lysates/30 µg protein), IP (MCF-7 WT cell lysate) and IF (MCF-7 WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) 57045 detected a band of appr. 58 in WT cell lysate with no corresponding band observed in KO cell lysate. This pattern was consistent in both HAP1 and MCF-7 cell lysate. A band was detected following IP (10 µl Ab used), this band was markedly more intense compared to band in input extract (6% of starting material) and no band was detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate. In IF (1:100 ab dilution), clear intense staining was observed only in WT cells.