Ubiquitin-specific protease 30, Abbexa, abx422618

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Ubiquitin-specific protease 30 (USP30) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 7 commercial research antibodies against human Ubiquitin-specific protease 30 (USP30).

The collection of antibodies was tested using MCF-7 WT and USP30 KO cells in the applications WB (WT and KO cell lysates/30 µg protein), IP (WT cell lysate) and IF (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) abx422618 detected a band of appr. 58 in WT cell lysate with no corresponding band observed in KO cell lysate. No band was detected following IP (2 µl Ab used). In immunofluorescence (IF; antibody dilution 1:1000), stronger staining was observed in WT cells compared to KO cells; however, background staining was also present.