Collagen triple helix repeat-containing protein 1, Thermo Fisher Scientific, MA5-29201

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Collagen triple helix repeat-containing protein 1 (CTHRC1) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 9 commercial research antibodies against human Collagen triple helix repeat-containing protein 1 (CTHRC1)

The collection of antibodies was tested using A-375 WT and CTHRC1 KD cells in the applications WB (WT and KD cell lysates/30 µg protein) and IP (WT cell lysate) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) MA5-29201 detected a faint band of appr. 26 kDa in WT cell lysate along with multiple intense bands of both higher and lower molecular weight. A band was detected following IP (2 µg Ab used), this band was markedly more intense compared to band in input extract (6% of starting material) and no band was detected in immunodepleted extract (6% of total unbound fraction), showing that the antibody does completely deplete the antigen from cell lysate.