Glucocorticoid receptor, Thermo Fisher Scientific, MA5-35309

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human NR3C1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 22 commercial research antibodies against human NR3C1.

The collection of antibodies was tested using A549 WT and NR3C1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein) and flow cytometry (KO and WT cells) according to YCharOS standard protocols (see reference).

In WB (1:500 ab dilution) MA5-35309 did not detect a band specific to WT cell lysate. Flow cytometry using MA5-35309 (1:2000 ab dilution) showed overlapping fluorescence intensity distributions for WT and KO cells, with no visible shift between the two populations.