Glucocorticoid receptor, Abcam, ab302677

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human NR3C1 and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 22 commercial research antibodies against human NR3C1.

The collection of antibodies was tested using A549 WT and NR3C1 KO cells in the applications WB (WT and KO cell lysates/20 µg protein) and flow cytometry (KO and WT cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab302677 detected a double band between 80-95 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Flow cytometry using ab302677 (1:8990 antibody dilution) showed a right-shifted fluorescence intensity distribution in WT cells compared with KO cells. The KO population partially overlapped with the secondary-only control, suggesting that a fraction of the signal may reflect non-specific binding or background staining.