PKN2, Abcam, ab138514

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Serine/threonine-protein kinase N2 (PKN2) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Serine/threonine-protein kinase N2 (PKN2).

The collection of antibodies was tested using HCT116 WT and PKN2 KO cells in the applications WB (WT and KO cell lysates/30 µg protein) and flow cytometry (KO and WT cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) ab138514 detected multiple intense bands (>10) none of which were specific to WT cell lysate. Flow cytometry using ab138514 (1:1000 ab dilution) showed overlapping fluorescence intensity distributions for WT and KO cells, with no visible shift between the two populations.