Guanine nucleotide-binding protein G(q) subunit alpha, Abclonal, A21111

Our team has carried out deep research in the published scientific literature to identify papers and reports using antibodies directed against human Guanine nucleotide-binding protein G(q) subunit alpha (GNAQ) and followed up by extraction and evaluation of the available data to assess its performance according to pAbmAbs rating criteria.

YCharOS is here conducting a large head-to-head study comparing 11 commercial research antibodies against human Guanine nucleotide-binding protein G(q) subunit alpha (GNAQ)

The collection of antibodies was tested using HAP1 WT and GNAQ KO cells in the applications WB (WT and KO cell lysates/30 µg protein) and flow cytometry (WT and KO cells) according to YCharOS standard protocols (see reference).

In WB (1:1000 ab dilution) A21111 detected a band of appr. 42 kDa in WT cell lysate with no corresponding band observed in KO cell lysate. Furthermore, faint bands of higher molecular weight were observed in both genotypes. Flow cytometry using A21111 (1:2000 ab dilution) showed overlapping fluorescence intensity distributions for WT and KO cells, with no visible shift between the two populations.