eIF5A, Abcam, ab32407

I am sharing a pAbmAbs generated literature review of KO validated antibodies. Here citing Manjunath et al. Cell reports, 2019. Manjunath et al. validate this antibody for detection of human eIF4A by Western blotting of HCT116 cell lysates. KO cell lines were made using lentiviral delivery of Cas9 and sgRNAs. A band was detected in NT, whereas no band was observed in eIF4A KO HCT116 cells (Figure 1c). Full lane images were not identified, why assessment of full blot cannot be done. Based on the available data, we have assigned a four-star rating, but we highly encourage other scientists to share their observations.