15 Lipoxygenase 1, Abcam, ab80221

In Chang et al., Biochemistry and Biophysics Reports; 46:102534 (2026), the authors investigate the role of ALOX15, encoding 15 Lipoxygenase 1 (Alox15), in lung fibrosis using siRNA-mediated KD in a bleomycin-induced pulmonary fibrosis mouse model. Abcam ab80221 is used for IHC of formalin-fixed, paraffin-embedded mouse lung sections following Alox15 KD, compared with a non-targeting siRNA control. Antibody dilution not provided. Data are shown in Figure 5G. Staining and the quantified positive area are reduced following ALOX15 KD, supporting specific detection of 15 Lipoxygenase 1/Alox15 by ab80221 in IHC under the tested conditions. However, changes in inflammation and tissue composition may also contribute to the reduced staining, limiting the assessment of specificity. We have rated ab80221 based on the available information and according to the pAbmAbs criteria.