15 Lipoxygenase 1, Abcam, ab244205

In Chang et al., Biochemistry and Biophysics Reports; 46:102534 (2026), the authors investigate the role of ALOX15, encoding 15 Lipoxygenase 1, in lung fibrosis using siRNA-mediated knockdown in a bleomycin-induced pulmonary fibrosis mouse model. Abcam ab244205 (dilution not reported) is used for WB of mouse lung lysates following Alox15 knockdown, compared with a non-targeting siRNA control (protein amount not reported). Data are shown in Figure 5F. The detected band is reduced following Alox15 knockdown (GAPDH loading control). This supports detection of 15 Lipoxygenase 1 by ab244205 in WB, although changes in inflammation and tissue composition may also contribute to the reduced signal. The blot is cropped and lacks molecular weight markers, limiting assessment of band size and nonspecific bands. We have rated ab244205 based on the available information and according to the pAbmAbs criteria.