VEGFR2, Abcam, ab134191

In Wang et al., NAR Cancer; 8 (2026), the authors study siRNA-based suppression of immune suppression and angiogenesis in melanoma. Abcam ab134191 is used for WB of human MDA-MB-231 cell lysates following siRNA VEGFR2 KD, compared with untreated and non-targeting siRNA controls Protein amount and antibody dilution not reported. Data are shown in Figure 3B. The detected band decreases with increasing siRNA concentration, supporting specific detection of VEGFR2 by ab134191 in WB under the tested conditions. The authors describe total-protein normalization, but no loading control is shown. The blot is cropped and lacks molecular weight markers, limiting assessment of band size, sample loading and nonspecific bands. We have rated ab134191 based on the available information and according to the pAbmAbs criteria.