TGF beta 1, Abcam, ab215715

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. TGFB1 encoding Transforming Growth Factor Beta 1 (TGF-beta-1) is the 7th most studied gene on the list. In Wang et al., NAR Cancer; 8 (2026), the authors investigate TGF-β1 silencing in cancer therapy. Abcam ab215715 is used for WB of human PANC-1 and MDA-MB-231 cell lysates following siRNA TGFB1 KD, compared with untreated and non-targeting siRNA control (protein amount not reported). Data are shown in Figure 3A. The detected band decreases with increasing siRNA concentration in both cell lines, supporting specific detection of TGF-β1 by ab215715 in WB under the tested conditions. The authors describe total-protein normalization, but no loading control is shown. The blot is cropped and lacks molecular weight markers, limiting assessment of band size, sample loading and nonspecific bands. We have rated ab215715 based on the available information and according to the pAbmAbs criteria.

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. TGFB1 encoding Transforming Growth Factor Beta 1 (TGF-beta-1) is the 7th most studied gene on the list. In Li et al., Bone Joint Res; 15(5):549–565 (2026), the authors study the role of TGF-β1 in bone repair. Abcam ab215715 (1:1000) is used for WB of rat bone marrow-derived endothelial progenitor cell lysates following TGFB1 knockdown with three different siRNAs, compared with a non-targeting siRNA control (protein amount not reported). Data are shown in Figure 4D. The detected band is reduced with two of the three TGFB1 siRNAs. GAPDH provides a loading control. This supports specific detection of TGF-β1 by ab215715 in WB under the tested conditions. However, the blot is cropped and lacks molecular weight markers, limiting assessment of band size and nonspecific bands. We have rated ab215715 based on the available information and according to the pAbmAbs criteria.