AT1R, Proteintech, 66415-1-Ig

pAbmAbs reviewed the publication entitled “Validation of six commercially available angiotensin II type 1 receptor antibodies”, in which Bingjie Li et al. evaluated Proteintech 66415-1-Ig against the angiotensin II (Ang II) type 1 receptor (AT1R) in WB and IF. The antibody was tested using AT1R global knockout SD rats, cardiomyocyte conditional knockout C57BL/6N mice, human AT1R-overexpressing CHO stable-transformed cell lines and human AT1R-overexpressing HEK293 cells. AT1R expression and localization were assessed through receptor-ligand binding assays, RT-PCR, WB, and IF. The WB analysis of 66415-1-Ig is presented in Figure 1. Only very narrowly cropped blots are shown. Uncropped blots are not available. The signal from 66415-1-Ig is unchanged in knockouts and up upon overexpression. Thus, based on the available data and our rating criteria, no more than a 1-star rating can be granted. The IF analysis of 66415-1-Ig is presented in Figure 2. No specific AT1R signal could be detected in WT cardiomyocytes vs KO or in overexpressing CHO cells. Thus a 1-star rating is granted. We encourage other scientists to share their observations.