Estrogen receptor alpha, Santa Cruz Biotechnology, sc-542

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. ESR1 encoding estrogen receptor alpha (ERα) is the ninth most studied gene on the list. In Dominguez and Micevych, Journal of Neuroscience (2010), the authors test MC-20 (sc-542) by WB (1:1000) using hypothalamic lysates (50 µg) from female WT and homozygous Esr1 KO mice. WT ovary lysate provides a positive control. The data are shown in Figure 2c. The antibody detects ~52- and ~66-kDa bands in WT hypothalamus that are absent in KO tissue, supporting specific detection of target under the tested conditions. Additional bands persist in KO tissue. No loading control is shown alongside this panel. We have rated sc-542 based on the available information and according to the pAbmAbs criteria. We highly encourage other scientists to share their observations.

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. ESR1 encoding estrogen receptor alpha (ERα) is the ninth most studied gene on the list. In Chen et al., Molecular and Cellular Biochemistry (2009), the authors generate an Esr1 KO mouse model using ACTB-Cre. MC-20 (sc-542) is used for WB of uterine lysates (80 µg) and IHC of paraffin-embedded uterus sections (ab dilution 1:400) comparing WT and KO. In WB, bands corresponding to full-length ERα (~65 kDa) and a shorter isoform (~46 kDa) are present in WT samples but absent in complete KO samples, with GAPDH as a loading control (Figure 2c). WT nuclear IHC staining is also absent in complete KO tissue (Figure 2d). Data supports specific detection of ERα in WB and IHC under the tested conditions, although additional WB bands and diffuse IHC background are observed. We have rated sc-542 based on the available information and according to the pAbmAbs criteria. We highly encourage other scientists to share their observations.