Estrogen receptor alpha, Millipore, 06-935

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. ESR1 encoding estrogen receptor alpha (ERα) is the ninth most studied gene on the list. In Glont et al., PLOS One (2019), the authors compare 06-935 head-to-head with two other ERα antibodies using ChIP-seq and RIME (IP–MS). Both assays use nuclear/chromatin preparations from crosslinked MCF7 cells, with IgG controls. ChIP-seq (10 µg antibody) shows similar ERα binding profiles across antibodies, with enrichment of the estrogen response element. Only two peaks are detected in ERα-negative MDA-MB-231 cells, indicating low background (Figure 1). RIME confirms capture of ERα and known associated proteins, with similar performance across antibodies (Figure 2). These data support specific enrichment of ERα in ChIP and RIME (IP–MS) under the tested conditions. No KO/KD controls are included. We highly encourage other scientists to share their observations.