Estrogen receptor alpha, Santa Cruz Biotechnology, sc-8002

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. ESR1 encoding estrogen receptor alpha (ERα) is the ninth most studied gene on the list. In Kavlashvili et al., PLOS One February 9, 2016, the authors use CRISPR-Cas9 to disrupt ESR1 in human ECC1 endometrial cancer cells. Santa Cruz sc-8002 is used for WB to compare ERα expression in parental cells and six independently isolated edited clones. The data is shown in Figure 1. The antibody detects a strong band in parental ECC1 cells that is not detected in five of six edited clones. β-actin serves as a loading control. These results support specific detection of ERα by sc-8002 in WB under the tested conditions. However, the ERα blot is cropped and lacks molecular-weight markers, limiting assessment of band size and additional nonspecific bands. We have rated sc-8002 based on the available information and according to the pAbmAbs criteria. We highly encourage other scientists to share their observations.