NFKBI, Santa Cruz Biotechnology, sc-114

We generated a top 1000 list of the most studied and most published proteins and analyzed literature validation data for commercially available research antibodies. NFKB1 is the seventh most studied gene on the list. In Herkenham et al., Journal of Neuroinflammation 8:141 (2011), the authors tested antibodies targeting NFKB1 p50 by Western blot using tissue homogenates from WT and Nfkb1 knockout mice. The data for sc-114 are shown in Figure 3c, using 50 µg of mouse brain homogenate per lane. The antibody detects multiple bands in WT and knockout sample, no bands were specifically detected in the WT homogenate. We have rated sc-114 based on the available information and according to the pAbmAbs criteria. We highly encourage other scientists to share their observations. This antibody product has been discontinued.